Nuwacell® fluorescent protein labeled hiPSC (scientific research-grade), originally developed by Nuwacell Biotechnologies Co., Ltd. is generated by insertion of reporter into the target sites using CRISPR-Cas9 technology. These constructed hiPSC can proliferate normally and express fluorescent protein stably for a long-time culture. More importantly, the afore-mentioned hiPSC displayed a typical human embryonic stem cells (hESCs)-like morphology, expressed hESCs pluripotency markers, maintained a normal karyotype, and had capacity to form three germ layers in vitro and in vivo, thus can be used in various stem cell related research and application.
Nuwacell Biotechnologies Co., Ltd. has a variety of different hiPSC cell lines (scientific research-grade), please call 400-888-3920 for detailed consultation.
Product manual download| Contents | Amount | Cat. No. | Introduction |
Storag |
|
Nuwacell® hiPSC-EGFP |
>2×10⁶ |
RC01004 |
Expressing green fluorescence protein (cytoplasmic). |
Liquid nitrogen |
|
Nuwacell® hiPSC-Luc-GFP |
>2×10⁶ |
RC01010 |
Expressing Luciferase and green fluorescence protein (nuclear). | |
|
Nuwacell® hiPSC-mCherry |
>2×10⁶ |
RC01016 |
Expressing red fluorescence protein (cytoplasmic). |
hiPSC-EGFP (cytoplasmic localization)（RC01004） 
(A-C) Cell morphology of long-term single-cell passaging of hiPSC-EGFP (P30). Scale bar, 200 μm.
(D)Representative flow cytometry analysis of pluripotency cell surface markers SSEA4 and TRA-1-81 in hiPSC-EGFP (P30).
(E) Representative quantitative PCR analysis of pluripotency markers POU5F1 and NANOG in hiPSC-EGFP (P30).
(F) Karyotyping of hiPSC-EGFP (P30) shows normal karotypes.
hiPSC-Luc-EGFP（RC01010） 
(A-C) Cell morphology of long-term single-cell passaging of hiPSC-Luc-EGFP (P30). Scale bar, 200 μm.
(D)Representative flow cytometry analysis of pluripotency cell surface markers SSEA4 and TRA-1-81 in hiPSC-Luc-EGFP (P30).
(E) Representative quantitative PCR analysis of pluripotency markers POU5F1 and NANOG in hiPSC-Luc-EGFP (P30).
(F) Karyotyping of hiPSC-Luc-EGFP (P30) shows normal karotypes.
hiPSC-mCherry（RC01016） 
(A-C) Cell morphology of long-term single-cell passaging of hiPSC-mCherry (P30). Scale bar, 200 μm.
(D)Representative flow cytometry analysis of pluripotency cell surface markers SSEA4 and TRA-1-81 in hiPSC-mCherry (P30).
(E) Representative quantitative PCR analysis of pluripotency markers POU5F1 and NANOG in hiPSC-mCherry (P30).
(F) Karyotyping of hiPSC-mCherry (P30) shows normal karotypes.
