Nuwacell® hiPSC（Male / Female）（RC01001）is reprogrammed from healthy human somatic cells using non-integrative reprogramming vectors. hiPSC can proliferate stably and rapidly for a long time in the Nuwacell hPSC culture system. More importantly, the cultured hiPSC displayed a typical human embryonic stem cells (hESCs)-like morphology, expressed hESCs pluripotency markers, maintained a normal karyotype, and had capacity to form three germ layers in vitro and in vivo, thus can be used in various stem cell related research and application.
- ✔ High-quality, “Footprint-free” hiPSC.
- ✔ Episomal reprogramming method, transgene-free.
- ✔ Long-term stable proliferation, and maintain normal karyotype.
- ✔ Strict quality inspection, and small differences between batches.
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Cell morphology 
The morphology of hiPSC (A, RC01001-A; B, RC01001-B) cultured at day 1, 2, and 3. Scale bar, 200 μm.
Cell surface markers 
Representative flow cytometry analysis of pluripotency cell surface markers (SSEA4 and TRA-1-81) in hiPSC (A, RC01001-A; B, RC01001-B).
Gray line: isotype control; Black line: antigen staining.
Pluripotency genes expression 
Representative quantitative PCR analysis of pluripotency markers (POU5F1 and NANOG) in hiPSC (A, RC01001-A; B, RC01001-B).
Teratoma 
Detection of the three-germ line lineages differentiation potential of hiPSC (A, RC01001-A; B, RC01001-B) in severe combined immunodeficient mice on 9 weeks. HE staining of teratoma showing neural tubes (ectoderm, left panels), cartilage (mesoderm, middle panels), and gut epithelia (endoderm, right panels). Scale bar, 200 μm.
5、STR 
Citations & References -
Establishment of Human Pluripotent Stem Cell-Derived Skin Organoids Enabled Pathophysiological Model of SARS-CoV-2 Infection2021.Jie Ma, et al.Advanced Science(IF 17.521).
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A splenic-targeted versatile antigen courier iPSC wrapped in coalescent erythrocyte-liposome as tumor nanovaccine.2021.Yuewen Zhai,et al.Science Advances(IF 14.957).
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Tfcp2l1 safeguards the maintenance of human embryonic stem cell self-renewal.2018. Sun, Hongwei, et al. Journal of Cellular Physiology(IF 6.513).
