Nuwacell® primary human mesenchymal stem cells (hMSC) （RC02003）can proliferate stably without microbial contamination, have normal karyotypes, express specific cell surface factors (CD73+ / CD90+ / CD105+, CD14- / CD34- / CD45- / CD79α- / HLA-DR-), and maintain the three-germ line lineages differentiation potential. Nuwacell® hMSC is suitable for various in vitro experiments, drug screening and safety evaluation, and cell regenerative medicine.
- ✔ Low generation, strong proliferation.
- ✔ Cell recovery efficiency≥90%.
- ✔ Cell differentiation potential.
| Contents | Amount | Cat. No. |
Storage |
|
Nuwacell® Human uc-MSC |
1×10⁶ | RC02003 | Liquid nitrogen |
Cell morphology 
The morphorlogy of hMSC cultured at day 3 after passaging (at passage 3, 4, and 5) in ncMission hMSC Medium.
Cells were plated into a T25 culture flask at a density of 6,000 cells/cm2. Scale bar, 200 μm.
Cell surface markers 
Representative flow cytometry analysis of cell surface markers (CD73+ / CD90+ / CD105+, CD14- / CD34- / CD45- / CD79α- / HLA-DR-), in hMSC at passage 5.
Gray line: isotype control; Black line: antigen staining.
Differentiation potential 
Differentiation potential of hMSC (P5) in ncMission hMSC Medium.
(A) Adipogenic differentiation (oil red staining), (B) Osteogenic differentiation (alizarin red staining), and (C) Cartilage differentiation (alixin blue staining). Scale bar, 200 μm.
Karyotype 
Karyotype analysis of primary hMSC (P5) was performed on G-banded metaphase chromosomes.
The result shows a normal 46, XY. At least 5 metaphases were examined with approximate resolution of 400 bands per haploid genome.
Citations & References -
Bioenergetic Crosstalk between Mesenchymal Stem Cells and various Ocular Cells through the intercellular trafficking of Mitochondria2020. Dan Jiang,et al. Theranostics(IF 11.6).
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Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro2022. Sang Luo, et al.Annals of Translational Medicine(IF 3.616).
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Dispersible and dissolvable porous microcarrier tablets enable efficient large scale hMSC expansion2020. Xiaojun Yan, et al. Tissue Engineering Part C: Methods(IF 3.273).
