Nuwacell Biotechnologies Co., Ltd. can provide customized hPSC-derived retinal pigment epithelial (RPE) cells based on the customer needs and requirements.
Nuwacell Biotechnologies Co., Ltd. has mastered the stable and efficient differentiation of hPSC-derived RPE cells technology. Nuwacell® hPSC-RPE cells are high-purity (>95% MiTF+/ZO-1+) retinal pigment epithelial cells from human pluripotent stem cells (hPSC). Nuwacell® hPSC-RPE cells can be used in related in vitro experiments, drug screening and safety evaluation, and cell transplantation experiments in disease animal models.
Nuwacell Biotechnologies Co., Ltd. complete the preparation, identification, storage, packaging, logistics and other related services with strict quality standards to meet the scientific research requirements of customers, which ensure the repeatability and reliability of subsequent experiments.
Flow chart 
Schematic of the hPSC-derived RPE cells differentiation using a Nuwacell® hPSC-RPE Cells Differentiation Kit.
RPE cells morphology and growth during differentiation 
The morphology of hPSC-derived RPE cells cultured at day 0 (hPSC), day 6 (RPE progenitors), day 16 (immature RPE), and day 45 (mature RPE) during differentiation. Scale bar, 200 μm.
hPSC-derived RPE cells specific markers expression 
Immunofluorescence staining of MiTF and ZO-1 for differentiated RPE cells at day 45 using a Nuwacell® hPSC-RPE Differentiation Kit. Scale bar, 200 μm.
Representative flow cytometry analysis of MiTF and ZO-1 for differentiated RPE cells at day 45 using a Nuwacell® hPSC-RPE Differentiation Kit.
Quantitative PCR of MiTF, ZO-1, RPE65, BEST1, MERTK, and PEDF for mature RPE cells at day 45 using a Nuwacell® hPSC-RPE Differentiation Kit. Scale bar, 200 μm.
