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RPE Differentiation of hPSC

Nuwacell Biotechnologies Co., Ltd. can provide customized hPSC-derived retinal pigment epithelial (RPE) cells based on the customer needs and requirements.

 

Nuwacell Biotechnologies Co., Ltd. has mastered the stable and efficient differentiation of hPSC-derived RPE cells technology. Nuwacell® hPSC-RPE cells are high-purity (>95% MiTF+/ZO-1+) retinal pigment epithelial cells from human pluripotent stem cells (hPSC). Nuwacell® hPSC-RPE cells can be used in related in vitro experiments, drug screening and safety evaluation, and cell transplantation experiments in disease animal models.

 

Nuwacell Biotechnologies Co., Ltd. complete the preparation, identification, storage, packaging, logistics and other related services with strict quality standards to meet the scientific research requirements of customers, which ensure the repeatability and reliability of subsequent experiments.

 

For specific information, please refer to Nuwacell® hPSC-RPE Differentiation Kit and Nuwacell® hPSC-derived RPE instructions, and call 400-888-3920 for detailed consultation.

Flow chart

Schematic of the hPSC-derived RPE cells differentiation using a Nuwacell® hPSC-RPE Cells Differentiation Kit.

RPE cells morphology and growth during differentiation

 

The morphology of hPSC-derived RPE cells cultured at day 0 (hPSC), day 6 (RPE progenitors), day 16 (immature RPE), and day 45 (mature RPE) during differentiation. Scale bar, 200 μm.

hPSC-derived RPE cells specific markers expression

 

Immunofluorescence staining of MiTF and ZO-1 for differentiated RPE cells at day 45 using a Nuwacell® hPSC-RPE Differentiation Kit. Scale bar, 200 μm.

 

 

Representative flow cytometry analysis of MiTF and ZO-1 for differentiated RPE cells at day 45 using a Nuwacell® hPSC-RPE Differentiation Kit.

Quantitative PCR of MiTF, ZO-1, RPE65, BEST1, MERTK, and PEDF for mature RPE cells at day 45 using a Nuwacell® hPSC-RPE  Differentiation Kit. Scale bar, 200 μm.

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