Nuwacell® ncEpic hPSC Medium（RP01001）is a xeno-free, feeder-free, and chemical-defined medium specially designed for the maintenance and expansion of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). hESC/hiPSCs can maintain the three-germ line lineages differentiation potential and self-renewal ability in ncEpic hPSC Medium, ensuring the repeatability and reliability of scientific research experiments. ncEpic can be used with Corning Matrigel, Vitronectin and Laminin recombinant human protein.
According to the third-party inspection company, ncEpic is free of bacteria, fungi, and mycoplasma.
- ✔ Serum-free, feeder-free.
- ✔ Chemical-defined.
- ✔ Supports robust expansion of hPSC.
Product manual download| Contents | Amount | Cat. No. | Storage |
|
Nuwacell® ncEpic hPSC Medium* |
500mL/Kit |
RP01001 | - |
|
ncEpic Basal Medium |
496mL |
RP01001 -01 | 2℃~8℃ |
|
ncEpic 125x Supplement |
4 mL |
RP01001-02 | -20℃~-80℃ |
* The complete ncEpic hPSC Medium can be stored at 2-8°C for up to 2 weeks.
1、Cell morphology 
hiPSCs growing inncEpic hPSC Medium on Matrigel for 2 days (A), 3 days (B), and 4 days (C).
Enlarged bright-field images of (C) are shown in D.Scale bar: 200 μm.
2、Single-cell passaging 
The morphology of long-term single-cell passaging of hiPSC (Nuwacell® hiPSC-Male, RC01001-A, P20) in ncEpic.
The morphology of hiPSC cultured at day 1 (E), 5 (F), and 10 (G) after passaging. Scale bar, 200 μm.
3、Cell surface markers 
Representative flow cytometry analysis of hiPSC (Nuwacell® hiPSC-Male, RC01001-A, P20) cell surface markers SSEA4 and TRA-1-81.
Gray line: isotype control; Black line: antigen staining.
4、Pluripotency genes expression 
Representative qPCR analysis of hiPSC (Nuwacell® hiPSC-Male, RC01001-A, P20) pluripotency markers OCT4 and NANOG.
5、Karyotype 
hPSCs (Nuwacell® hiPSC-Male, RC01001-A, P20) Maintained in ncEpic Display a Normal Karyotype.
Citations & References -
Base editing-mediated splicing correction therapy for spinal muscular atrophy2020. Xiang Lin, et al. Cell Research(IF 46.297).
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Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice2019.Jinjing Li，et al.National Science Review(IF 23.178).
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ESC-sEVs Rejuvenate Senescent Hippocampal NSCs by Activating Lysosomes to Improve Cognitive Dysfunction in Vascular Dementia2020. Guowen Hu, et al. Advanced Science(IF 17.521).
