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Reagents
ncEpic hiPSC/hESC Medium
ncEpic hiPSC/hESC Medium
ncEpic hiPSC/hESC Medium
ncEpic hiPSC/hESC Medium

Nuwacell® ncEpic hPSC Medium(RP01001)is a xeno-free, feeder-free, and chemical-defined medium specially designed for the maintenance and expansion of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). hESC/hiPSCs can maintain the three-germ line lineages differentiation potential and self-renewal ability in ncEpic hPSC Medium, ensuring the repeatability and reliability of scientific research experiments. ncEpic can be used with Corning Matrigel, Vitronectin and Laminin recombinant human protein.

According to the third-party inspection company, ncEpic is free of bacteria, fungi, and mycoplasma.

Advantage
  • ✔ Serum-free, feeder-free.
  • ✔ Chemical-defined.
  • ✔ Supports robust expansion of hPSC.
Contents Amount Cat. No. Storage

Nuwacell® ncEpic hPSC Medium*

500mL/Kit

RP01001 -

ncEpic Basal Medium

496mL

RP01001 -01 2℃~8℃

ncEpic 125x Supplement

4 mL

RP01001-02 -20℃~-80℃

* The complete ncEpic hPSC Medium can be stored at 2-8°C for up to 2 weeks.

1、Cell morphology

 

hiPSCs growing inncEpic hPSC Medium on Matrigel for 2 days (A), 3 days (B), and 4 days (C).

Enlarged bright-field images of (C) are shown in D.Scale bar: 200 μm.

 

 

2、Single-cell passaging

 

The morphology of long-term single-cell passaging of hiPSC (Nuwacell® hiPSC-Male, RC01001-A, P20) in ncEpic.

The morphology of hiPSC cultured at day 1 (E), 5 (F), and 10 (G) after passaging. Scale bar, 200 μm.

 

 

3、Cell surface markers

Representative flow cytometry analysis of hiPSC (Nuwacell® hiPSC-Male, RC01001-A, P20) cell surface markers SSEA4 and TRA-1-81.

Gray line: isotype control; Black line: antigen staining.

 

4、Pluripotency genes expression

Representative qPCR analysis of hiPSC (Nuwacell® hiPSC-Male, RC01001-A, P20) pluripotency markers OCT4 and NANOG.

5、Karyotype

hPSCs (Nuwacell® hiPSC-Male, RC01001-A, P20) Maintained in ncEpic Display a Normal Karyotype.

 

Citations & References
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