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Reagents
ncLaminin511
ncLaminin511

Laminin(RP01025), composed of three peptide chains of α, β, and γ, is an important part of the extracellular matrix. Moreover, laminin is also an intermediary for certain cell-cell interactions, which plays an important role in regulating cell functions.

Nuwacell® ncLaminin511, originally developed by Nuwacell Biotechnologies Co., Ltd. is an improved recombinant laminin with defined formulation and no foreign components, which can provide a defined surface for adherent culture of human pluripotent stem cells (hPSC), neuons, and other cells.

Contents Amount Cat. No. Storage

Nuwacell® ncLaminin511

1mL(100ug/mL)

RP01025 -20°C~-80℃
hPSC morphology

 

ncLaminin511 supports long-term single-cell passaging of hiPSC attachment and survival when used in conjunction with ncEpic.

(A) The morphology of hiPSC cultured at day 8 after passaging (P30). (B) The enlargerd panel B is a magnified view of A. Scale bar, 200 μm.

 

 

ncLaminin511 supports multiple cell lineages of hiPSC attachment and survival when used in conjunction with ncEpic.

The morphology of multiple cell lineages of hiPSC (RC01001-A, RC01001-B, H9, and NCB19001) at day 4 after passaging. Scale bar, 200 μm.

 

 

Comparison of cell adhesion effects on different substrates for single-cell passaging of hiPSC. Statistics of the number of colonies cultured on day 6 after passaging. Cells were plated into individual wells of a 6-well plate at a density of 1,000 cells/well.

ncLaminin511 is significantly better than other substrates.

2、Neuron morphology

 

ncLaminin511 supports long-term culture of neuron attachment and survival. Scale bar, 200 μm.

 

Citations & References
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