Laminin（RP01025）, composed of three peptide chains of α, β, and γ, is an important part of the extracellular matrix. Moreover, laminin is also an intermediary for certain cell-cell interactions, which plays an important role in regulating cell functions.
Nuwacell® ncLaminin511, originally developed by Nuwacell Biotechnologies Co., Ltd. is an improved recombinant laminin with defined formulation and no foreign components, which can provide a defined surface for adherent culture of human pluripotent stem cells (hPSC), neuons, and other cells.
ncLaminin511 supports long-term single-cell passaging of hiPSC attachment and survival when used in conjunction with ncEpic.
(A) The morphology of hiPSC cultured at day 8 after passaging (P30). (B) The enlargerd panel B is a magnified view of A. Scale bar, 200 μm.
ncLaminin511 supports multiple cell lineages of hiPSC attachment and survival when used in conjunction with ncEpic.
The morphology of multiple cell lineages of hiPSC (RC01001-A, RC01001-B, H9, and NCB19001) at day 4 after passaging. Scale bar, 200 μm.
Comparison of cell adhesion effects on different substrates for single-cell passaging of hiPSC. Statistics of the number of colonies cultured on day 6 after passaging. Cells were plated into individual wells of a 6-well plate at a density of 1,000 cells/well.
ncLaminin511 is significantly better than other substrates.
ncLaminin511 supports long-term culture of neuron attachment and survival. Scale bar, 200 μm.
Generation of induced pluripotent stem cell PLAFMCi002-A derived from peripheral blood mononuclear cells of polycystic kidney disease patient with PKD1 mutation2020. Tao Wang , et al. Stem Cell Research(IF 1.587).
Generation of an induced pluripotent stem cell line BIOi002-A from a patient with autosomal dominant optic atrophy2021. Xiaohui Zhang , et al. Stem Cell Research(IF 1.587).
Generation of an induced pluripotent stem cell line HUSTTJi001-A from a Moyamoya disease patient with RNF213 gene mutation2021. Zhijuan Mao , et al. Stem Cell Research(IF 1.587).