hPSC Nucleofection Buffer
hPSC Nucleofection Buffer
Nuwacell® hPSC Nucleofection Buffer（RP01005），originally developed by Nuwacell Biotechnologies Co., Ltd. is a a high-efficiency medium specially developed for electrotransfection of human pluripotent stem cells (iPSC, ESC) cultured under various undifferentiated conditions. This system can achieve stable and effective transfection, high cell survival rate, and low side-effect on downstream experiments.
Advantage
- ✔ Adapted to LONZA Nucleofector system.
- ✔ Stable and effective transfection.
- ✔ High cell survival rate.
Product manual download| Contents | Amount | Cat. No. | Storage |
|
Nuwacell® hPSC Nucleofection Buffer |
2.5mL(25rnx) | RP01005 | -2℃~8℃ |
1、Transfection of hiPSC 
Example of hPSC Nucleofection Buffer transfection of hiPSC：
Electroporation 2μg of pPBmPNEE plasmid (~6.5kb) expressing EGFP into hiPSC, and detection EGFP-positive cells at 24 hours after transfection using flow cytometry.
(A) Negative control with no plasmid added during electroporation. (B) pPBmPNEE plasmid added during electroporation. The transfection efficiency can reach 85.43%.
Example 
The cell morphology of the Nuwacell® hiPSC-Luc-GFP cell line prepared by electroporation with hiPSC/hESC electroporation solution.
(C, D) The morphology of hiPSC-Luc-GFP cultured at day 3 after passaging (at passage 32). Scale bar, 100 μm.
Citations & References
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Please call 400 888 3920 for more references.
