Nuwacell® ncMission hMSC Medium（RP02010）, originally developed by Nuwacell Biotechnologies Co., Ltd. is a serum-freeand animal component-free formulation designed for the maintenance of human mesenchymal stem cells (hMSCs). ncMission hMSC Medium provides full support for the long-term growth of hMSCs while maintaining the expression of MSC-specific surface markers (CD73+ / CD90 + / CD105+, CD14- /CD34- / CD45- / CD79a- / HLA-DR-) and trilineage differentiation potential.
According to the third-party inspection company, ncMission hMSC medium is free of bacteria, fungi, and mycoplasma, and the endotoxin content is less than 0.5 EU/mL.
- ✔ Serum-free, Xeno-free.
- ✔ Defined formulation.
- ✔ Suitable for primary culture and subculture.
| Contents | Amount | Cat. No. | Storage |
|
Nuwacell® ncMission hMSC Medium* |
1 Kit | RP02010 | - |
| ncMission Basal Medium | 500 mL | RP02010-01 | 2℃~8℃ |
| ncMission 21x Supplement | 25 mL | RP02010-02 | -20℃ ~ -80℃ |
1、Primary culture of hMSC 
Bright-field images of day 12 hMSCs after isolation from umbilical cord explants.
Small, medium and large sized colonies (left,middle and right) of hMSCs are formed. The hMSCs are isolated from 0.5 g Wharton’s Jelly and cultured in a T75 flask. Scale bar: 200 μm.
Comparison of ncMission hMSC Medium to commercial A medium. hMSCs are isolated from 0.5 g Wharton’s Jelly andcultured in a T75 flask. ncMission hMSC Medium provides better support for primary hMSC growth.
A) The number of hMSC colonies at Day 12. B) The total cell number at Day 12. C) The time of initial hMSC appearance.
2、Subculture of hMSC 
hMSCs maintain the typical morphologyover passages when cultured in ncMission hMSC Medium. Illustrations show the bright-field images of cells at day 3 of passage 3, 4, and 5. The seeding density is 6000/cm2 with 0.2 mL/cm2 medium. Scale bar:200 μm.
ncMission Medium allows better proliferation for hMSCs than commercial A medium.
D) The population doubling rate of hMSCs cultured in two mediums. E) The population doubling time of hMSCs cultured in two mediums.
3、Cell surface marker 
Representative flow cytometry analysis of cell surface markers (CD73+ / CD90+ / CD105+, CD14- / CD34- / CD45- / CD79α- / HLA-DR-), in hMSC (P5).
Gray line: isotype control; black line: antigen staining.
4、Differentiation potential 
Differentiation potential of hMSC (P5) in ncMission hMSC Medium.
(F) Adipogenic differentiation (oil red staining), (G) Osteogenic differentiation (alizarin red staining), and (H) Cartilage differentiation (alixin blue staining). Scale bar, 200 μm.
Citations & References -
A bone-targeted engineered exosome platform delivering siRNA to treat osteoporosis.2021. Yongzhi Cui, et al. Bioactive Materials(IF 16.874).
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Small extracellular vesicles secreted by human iPSC-derived MSC enhance angiogenesis through inhibiting STAT3dependent autophagy in ischemic stroke2020. Yuguo Xia, et al. Stem Cell Research & Therapy(IF 8.079).
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Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.2022. Sang Luo, et al.Annals of Translational Medicine(IF 3.616).
