Nuwacell® hPSC-RPE Differentiation Kit（RP01016）, originally developed by Nuwacell Biotechnologies Co., Ltd. is suitable for the differentiation of human pluripotent stem cells (hPSC) into RPE cells. This product includes RPE cells differentiation medium, RPE cells purification working solution, and RPE cells cryopreservation protection solution. High purity RPE (>95% MiTF+/ZO-1+) can be obtained using a hPSC-RPE Differentiation Kit, thus can be used in related in vitro experiments, drug screening and safety evaluation, and cell transplantation experiments in disease animal models.
Product manual download| Contents | Amount | Cat. No. | Storage |
|
Nuwacell® hPSC- RPE Differentiation Kit |
1 Kit | RP01016 |
Basal medium 2℃～8℃ Supplement -20℃～-80℃ |
1、Process 
Schematic of the hPSC-RPE cells differentiation using a hPSC-RPE Cells Differentiation Kit.
2、Cell morphology during differentiation 
The morphology of hPSC-RPE cells cultured at day 0 (hPSC), day 6 (RPE progenitors), day 16 (immature RPE), and day 45 (mature RPE) during differentiation. Scale bar, 200 μm.
3、Cell specific markers expression 
Immunofluorescence staining of MiTF and ZO-1 for differentiated RPE cells at day 45 using a hPSC-RPE Cells Differentiation Kit.
Scale bar, 200 μm.
Representative flow cytometry analysis of MiTF and ZO-1 for differentiated RPE cells at day 45 using a hPSC-RPE Cells Differentiation Kit.
Quantitative PCR of MiTF, ZO-1, RPE65, BEST1, MERTK, and PEDF for mature RPE cells at day 45 using a hPSC-RPE Cells Differentiation Kit. Scale bar, 200 μm.
Citations & References 
