Nuwacell® hPSC-Nephron Differentiation Kit（RP01015）, originally developed by Nuwacell Biotechnologies Co., Ltd. is suitable for the differentiation of human pluripotent stem cells (hPSC) into Kidney Organoid, which have a nephron-like structure after maturation. These differentiated nephron-like organoids efficiently express specific markers (e.g., CDH1, LTL, PODXL, etc.), thus are suitable for various in vitro experiments, drug screening and safety evaluation, and cell transplantation experiments in disease animal models.
Product manual download| Contents | Amount | Cat. No. | Storage |
|
Nuwacell® hPSC-Nephron Differentiation Kit |
1 Kit | RP01015 |
Basal medium 2℃～8℃ Supplement -20℃～-80℃ |
1、Process 
Schematic of the hPSC-derived nephron-like organoids differentiation using a hPSC-Nephron Differentiation Kit.
2、Cell morphology during differentiation from day 0 to 8 
The morphology of hPSC-derived nephron cultured at day 0, 4, 6, and 8 during differentiation. Scale bar, 200 μm.
3、Cell morphology during differentiation from day 8 to 20 (2D) 
(A, B) The morphology of hPSC-derived Nephron cultured at day 14 (A) and 20 (B) during differentiation. (C) The enlargerd panel C is a magnified view of B. Scale bar, 200 μm.
4、Cell morphology during differentiation from day 8 to 17 (3D) 
(D, E) The morphology of kidney organoids cultured at day 13 (D) and 17 (E) during differentiation. (F) The enlargerd panel F is a magnified view of E. Scale bar, 200 μm.
5、hPSC-derived Nephron specific markers expression 
Immunofluorescence staining of PODXL (sertoli cell specific marker), CDH1 (proximal tubule specific marker), LTL (distal tubule specific marker), and PAX8 (renal capsule specific marker) for 2-dimension culture of Nephron at day 20 using a hPSC-Nephron Differentiation Kit. Scale bar, 50 μm.
