Nuwacell® hPSC-Motor Neuron Differentiation Kit（RP01018）, originally developed by Nuwacell Biotechnologies Co., Ltd. is suitable for the highly effective differentiation of human pluripotent stem cells (hPSC) into motor neurons. The differentiated motor neurons can express motor neuron-specific markers (e.g., ChAT, MAP2, Synapyophysin, etc.), and have the characteristic electrophysiological activity. These differentiated motor neurons are suitable for in vitro studies and cell transplantation studies in disease animal models.
Product manual download| Contents | Amount | Cat. No. | Storage |
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Nuwacell® hPSC-Motor Neuron Differentiation Kit |
1 Kit | RP01018 |
Basal medium 2℃～8℃ Supplement -20℃～-80℃ |
1、Process 
Schematic of the hPSC-derived motor neuron differentiation using a hPSC-Motor Neuron Differentiation Kit.
2、Cell morphology during differentiation 
The morphology of hPSC-derived motor neuron cultured at day 4 (A), 9 (B), 12 (C), and 30 (D) during differentiation. Scale bar, 200 μm.
3、Cell specific markers of MNP 
Immunofluorescence staining of Olig2 and Tubulin for differentiated motor neuron progenitor (MNP) cells at day 9 using a hPSC-Motor Neuron Differentiation
Immunofluorescence staining of CHAT and βIII-Tubulin for differentiated mature motor neurons (mMN) at day 35 using a hPSC-Motor Neuron Differentiation Kit. Scale bar, 50 μm.
4、Cell specific markers expression during differentiation 
(A) Quantitative PCR of PAX6 and OLIG2 (MNP specific markers), during MNP differentiation from day 0 to day 9.
(B) Quantitative PCR of ISL1 and HB9 (postmitotic MN specific markers), during pMN differentiation from day 9 to day 12.
(C) Quantitative PCR of ChAT, MAP2 and Synapyophysin (mMN specific markers), during mMN differentiation from day 15 to day 40.
5、Excitability analysis of hPSC-mMN (at day 30) by electrophysiology 
A representative image of electrophysiological recording on individual mMN. Scale bar, 50 μm.
Inward sodium and outward potassium currents were reliably elicited from a polarized voltage in the voltage-clamp mode.
Representative traces of induced action potentials in the current-clamp mode.
