Nuwacell® hiPSC-Motor Neuron Progenitor（RC01009）, originally developed by Nuwacell Biotechnologies Co., Ltd. are motor neuron precursor cells generated from human induced pluripotent stem cells (hiPSC), and can be differentiated into mature motor neuron (mMN). The differentiated mMN can express motor neuron-specific markers (e.g., ChAT, MAP2, Synapyophysin, etc.), and have the characteristic electrophysiological activity. These differentiated motor neurons are suitable for in vitro studies and cell transplantation studies in disease animal models.
- ✔ hiPSC differentiated to MNP on day 8.
- ✔ Can differentiated to mMN.
- ✔ Cell recovery efficiency≥80%.
| Contents | Amount | Cat. No. | Storage |
|
Nuwacell® hiPSC-Motor Neuron Progenitor |
1×10⁶ | RC01009 | Liquid nitrogen |
Cell morphology 
The morphology of Nuwacell® hiPSC-MNP cultured at day 1, 3, 11, and 21 after cell recovery. Scale bar, 200 μm.
MNP specific markers expression 
Immunofluorescence staining of Olig2 and Tubulin for differentiated motor neuron progenitor (MNP) cells. Scale bar, 50 μm.
hiPSC- mMN specific markers expression 
Immunofluorescence staining of ChAT and βIII-Tubulin for differentiated mature motor neurons (mMN) at day 26 after cell recovery. Scale bar, 50 μm.
Excitability analysis of hiPSC-mMN 
A representative image of electrophysiological recording on individual mMN at day 21 after cell recovery. Scale bar, 50 μm.
Inward sodium and outward potassium currents were reliably elicited from a polarized voltage in the voltage-clamp mode.
Representative traces of induced action potentials in the current-clamp mode.
