Nuwacell Biotechnologies Co., Ltd. can provide customized hPSC-derived Nephron based on the customer needs and requirements.
Nuwacell Biotechnologies Co., Ltd. has mastered the stable and efficient differentiation of hPSC-derived Nephron technology. Nuwacell® hPSC-Nephron are high-quality nephron-like organoids generated from human pluripotent stem cells (hPSC). These differentiated nephron-like organoids efficiently express specific markers (e.g., CDH1, LTL, PODXL, etc.), thus are suitable for various in vitro experiments, drug screening and safety evaluation, and cell transplantation experiments in disease animal models.
Nuwacell Biotechnologies Co., Ltd. complete the preparation, identification, storage, packaging, logistics and other related services with strict quality standards to meet the scientific research requirements of customers, which ensure the repeatability and reliability of subsequent experiments.
For specific information, please refer to the instructions of Nuwacell® hPSC-Nephron Differentiation Kit and Nuwacell® hPSC-Nephron Progenitor Cells (NPC), and call 400-888-3920 for detailed consultation.
Process 
Schematic of the hPSC-derived nephron-like organoids differentiation using a Nuwacell® hPSC-Nephron Differentiation Kit.
Cell morphology during differentiation from day 0 to 8 
The morphology of hPSC-derived nephron cultured at day 0, 4, 6, and 8 during differentiation. Scale bar, 200 μm.
Cell morphology during differentiation from day 8 to 20 (2D) 
(A, B) The morphology of hPSC-derived Nephron cultured at day 14 (A) and 20 (B) during differentiation. (C) The enlargerd panel C is a magnified view of B. Scale bar, 200 μm.
Cell morphology during differentiation from day 8 to 17 (3D) 
(D, E) The morphology of kidney organoids cultured at day 13 (D) and 17 (E) during differentiation. (F) The enlargerd panel F is a magnified view of E. Scale bar, 200 μm.
hPSC-derived Nephron specific markers expression 
Immunofluorescence staining of PODXL (sertoli cell specific marker), CDH1 (proximal tubule specific marker), LTL (distal tubule specific marker), and PAX8 (renal capsule specific marker) for 2-dimension culture of Nephron at day 20 using a hPSC-Nephron Differentiation Kit. Scale bar, 50 μm.
