Nuwacell Biotechnologies Co., Ltd. can provide customized hPSC-derived motor neurons (MN) based on the customer needs and requirements.
Nuwacell Biotechnologies Co., Ltd. has mastered the stable and efficient differentiation of hPSC-derived MN technology. Nuwacell® hPSC-derived MN are high-purity motor neurons generated from human pluripotent stem cells (hPSC). The differentiated motor neurons can express motor neuron-specific markers (e.g., ChAT, MAP2, Synapyophysin, etc.), and have the characteristic electrophysiological activity. These differentiated motor neurons are suitable for in vitro studies and cell transplantation studies in disease animal models.
Nuwacell Biotechnologies Co., Ltd. complete the preparation, identification, storage, packaging, logistics and other related services with strict quality standards to meet the scientific research requirements of customers, which ensure the repeatability and reliability of subsequent experiments.
Flow chart 
Schematic of the hPSC-derived motor neuron differentiation using a Nuwacell® hPSC-Motor Neuron Differentiation Kit.
Motor neuron morphology and growth during differentiation 
The morphology of hPSC-derived motor neuron cultured at day 4, 9, 12, and 30 during differentiation. Scale bar, 200 μm.
hPSC-derived motor neuron specific markers expression 
Immunofluorescence staining of Olig2 and Tubulin for differentiated motor neuron progenitor (MNP) cells at day 9 using a Nuwacell® hPSC-Motor Neuron Differentiation Kit. Scale bar, 50 μm.
Immunofluorescence staining of CHAT and βIII-Tubulin for differentiated mature motor neurons (mMN) at day 35 using a Nuwacell® hPSC-Motor Neuron Differentiation Kit. Scale bar, 50 μm.
hPSC-derived motor neuron specific markers expression 
(A) Quantitative PCR of PAX6 and OLIG2 (MNP specific markers), during MNP differentiation from day 0 to day 9.
(B) Quantitative PCR of ISL1 and HB9 (postmitotic MN specific markers), during pMN differentiation from day 9 to day 12.
(C) Quantitative PCR of ChAT, MAP2 and Synapyophysin (mMN specific markers), during mMN differentiation from day 15 to day 40.
Excitability analysis of hPSC-derived mMN (at day 30) by electrophysiology 
A representative image of electrophysiological recording on individual mMN. Scale bar, 50 μm.
Inward sodium and outward potassium currents were reliably elicited from a polarized voltage in the voltage-clamp mode.
Representative traces of induced action potentials in the current-clamp mode.
