Nuwacell Biotechnologies Co., Ltd. can provide customized hPSC-derived midbrain dopaminergic progenitors (mDAP) based on the customer needs and requirements.
Nuwacell Biotechnologies Co., Ltd. has mastered the stable and efficient differentiation of hPSC-derived mDAP technology. Nuwacell® hPSC-derived mDAP are high-purity (>90% Lmx1a+/Foxa2+/En1+) midbrain dopaminergic progenitors generated from human pluripotent stem cells (hPSC).
Nuwacell® hPSC-derived mDAP can be used in scientific research on neurodegenerative diseases, drug screening, and cell transplantation of Parkinson's disease models.
Nuwacell Biotechnologies Co., Ltd. complete the preparation, identification, storage, packaging, logistics and other related services with strict quality standards to meet the scientific research requirements of customers, which ensure the repeatability and reliability of subsequent experiments.
For specific information, please refer to Nuwacell® hPSC-mDAP Differentiation Kit and Nuwacell® hPSC-mDAP instructions, and call 400-888-3920 for detailed consultation.
Schematic of the hPSC-mDAP differentiation using a Nuwacell® hPSC-Dopaminergic Neuron Differentiation Kit.
The morphology of hPSC-derived DA cultured at day 0 (hiPSC), day 11 (mDAP), day 12 (subculture of mDAP), and day 45 (mDAN) during differentiation. Scale bar, 200 μm.
Immunofluorescence staining of LMX1A, FOXA2, and EN1 for differentiated mDAP using a Nuwacell® hPSC-mDAP Differentiation Kit. Scale bar, 200 μm.
Quantitative PCR of NKX2.1 (a diencephalon marker), DBX1 (a midbrain marker), LMX1a (a midbrain marker), FOXA2 (a midbrain marker), EN1 (a midbrain marker), OTX2 (a midbrain marker), and GBX2 (a hindbrain marker).
Representative flow cytometry analysis of FOXA2/En1 and FOXA2/LMX1a.
Immunofluorescence staining of TH and Nurr1 for differentiated mDAN using a Nuwacell®hPSC-Dopaminergic Neuron Differentiation Kit. Scale bar, 200 μm.
Radioimmunoassay of dopamine release level of differentiated mDAN (at day 45), which is higher than that from hPSC control (i.e., >2000pg/mL versus <100pg/mL).
Excitability analysis of hPSC-derived mDAN (at day 45) by electrophysiology
A representative image of electrophysiological recording on individual mDAN. Scale bar, 20 μm. Representative traces of spontaneous action potentials at 2-10 Hz in the current-clamp mode.