Nuwacell Biotechnologies Co., Ltd. can provide customized hPSC-derived MSC based on the customer needs and requirements.
Nuwacell Biotechnologies Co., Ltd. has mastered the stable and efficient differentiation of hPSC-derived MSC technology. The derivation of hPSC-MSC can proliferate stably with normal karyotype, express specific cell surface markers (CD73+/ CD90+/ CD105+, CD14- / CD34- / CD45- / CD79α- / HLA-DR-), and harbour three-germ line lineages differentiation potential (osteogenesis, cartilage, and adipose differentiation), which can be used in related in vitro experiments, drug screening and safety evaluation, and cell transplantation experiments in disease animal models.
Nuwacell Biotechnologies Co., Ltd. complete the preparation, identification, storage, packaging, logistics and other related services with strict quality standards to meet the scientific research requirements of customers, which ensure the repeatability and reliability of subsequent experiments.
For specific information, please refer to the instructions of Nuwacell® hPSC-derived Mesenchymal Stem Cell Differentiation Kit and Nuwacell® hPSC-derived Mesenchymal Stem Cells, and call 400-888-3920 for detailed consultation.
Flow chart 
Schematic of the hPSC-derived MSC differentiation using a Nuwacell® hPSC-MSC Differentiation Kit.
MSC morphology and growth during differentiation 
The morphology of hPSC-MSC cultured at day 1, 3, 8, and 14 during differentiation. Scale bar, 200 μm.
MSC surface marker 
Representative flow cytometry analysis of cell surface markers (CD73+ / CD90+ / CD105+, CD14- / CD34- / CD45- / CD79α- / HLA-DR-), in MSC at passage 5. Negative control is represented in grey.
CD73 + / CD90 + / CD105 +，CD14－/ CD34－ / CD45－ / CD79α－/ HLA-DR－.
Three-germ line lineages differentiation 
Detection of the three-germ line lineages differentiation potential of MSC (at passage 5) in ncMission hMSC Medium.
(A) fat differentiation (oil red staining), (B) hard bone differentiation (alizarin red staining), and (C) cartilage differentiation (alixin blue staining). Scale bar, 200 μm.
