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Reprogramming

The various somatic cells can be reprogrammed into induced pluripotent stem cells (scientific research-grade) using non-integrative reprogramming vectors. These generated human iPSC displayed a typical human embryonic stem cells (hESCs)-like morphology, expressed hESCs pluripotency markers, maintained the same karyotypes to their parent cells, and had capacity to form three germ layers in vitro and in vivo.

 

The human cell research and development center of Nuwacell Biotechnologies Co., Ltd. was established in accordance with the current Good Manufacturing Practice (cGMP) standards, including 1000 m2 clinical grade cell laboratory (B+A level), 1000 m2 scientific research grade cell laboratory (C+A level), 220 m2 barrier type experimental animal room (C level) and 350 m2 molecular laboratory and other R&D and production platforms. The R&D center is equipped with a series of advanced cell R&D, production, and quality inspection equipment, and has established a complete set of standardized quality management system. Nuwacell Biotechnologies Co., Ltd. has successfully generated a number of scientific and clinical grade hiPSC cell lines, including healthy controls and disease patients, which can be used in related in vitro experiments, drug screening and safety evaluation, and cell transplantation experiments in disease animal models.

 

Nuwacell Biotechnologies Co., Ltd. complete the preparation, identification, storage, packaging, logistics and other related services with strict quality standards to meet the scientific research requirements of customers, which ensure the repeatability and reliability of subsequent experiments.

The morphorlogy of hiPSC colonies during reprogramming

 

The morphorlogy of human induced pluripotent stem cells (hiPSC) reprogrammed from peripheral blood cells.

Nuwacell Biotechnologies Co., Ltd. has rich experience and technical strength in the somatic cells reprogramming, and can obtain hundreds of cell colonies for each reprogramming experiment. As shown in the figure, there are 10 hiPSC colonies under one field of view, and a large number of clones ensure the high success rate of reprogramming. Scale bar, 200μm.

The morphorlogy of hiPSC colonies during expansion

 

(A) The morphology of hiPSC colonies during the initial stage of reprogramming.

(B) The morphology of hiPSC colonies during the expansion. Scale bar, 200μm.

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