The various somatic cells can be reprogrammed into induced pluripotent stem cells (scientific research-grade) using non-integrative reprogramming vectors. These generated human iPSC displayed a typical human embryonic stem cells (hESCs)-like morphology, expressed hESCs pluripotency markers, maintained the same karyotypes to their parent cells, and had capacity to form three germ layers in vitro and in vivo.

The human cell research and development center of Nuwacell Biotechnologies Co., Ltd. was established in accordance with the current Good Manufacturing Practice (cGMP) standards, including 1000 m² clinical grade cell laboratory (B+A level), 1000 m² scientific research grade cell laboratory (C+A level), 220 m² barrier type experimental animal room (C level) and 350 m² molecular laboratory and other R&D and production platforms. The R&D center is equipped with a series of advanced cell R&D, production, and quality inspection equipment, and has established a complete set of standardized quality management system. Nuwacell Biotechnologies Co., Ltd. has successfully generated a number of scientific and clinical grade hiPSC cell lines, including healthy controls and disease patients, which can be used in related in vitro experiments, drug screening and safety evaluation, and cell transplantation experiments in disease animal models.

Nuwacell Biotechnologies Co., Ltd. complete the preparation, identification, storage, packaging, logistics and other related services with strict quality standards to meet the scientific research requirements of customers, which ensure the repeatability and reliability of subsequent experiments.

(A) The morphology of hiPSC colonies during the initial stage of reprogramming.

(B) The morphology of hiPSC colonies during the expansion. Scale bar, 200μm.